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Peripheral blood smear showing microfilaria, Leishman stain

Author: Harsha M Dangare, MD, 12/30/2023
Category: Infectious Disease > Parasites > Filarasis
Published Date: 09/08/2025

•A 26-year old male, presented with fever, yellowish discoloration of eyes with suspected hepatitis. The patient sample for CBC was taken at 11 am, which showed the microfilariae on peripheral blood smear. The patient was native of filaria endemic area and has relocated 3 months back in search of work.

•Blood samples should be collected between 22:00 and 02:00 hours for nocturnally periodic forms of brugian and bancroftian filariasis. But when the parasite load is high they can be seen in day time also as in our case in which the patient sample was taken at 11 am which showed the microfilariae.

•Detecting microfilaria in peripheral blood with or without Diethylcarbamazine citrate provocation is the common diagnostic modality in suspected cases.

•Highly sensitive and specific filarial antigen detection assays by ELISA method. This test is positive in early stages of the disease when the adult worms are alive and becomes negative once they are dead. DNA probes using Polymerase Chain Reaction (PCR)test is of high specificity and sensitivity to  detects parasite DNA in humans and vectors in both bancroftian and brugian filariasis.

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